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首頁(yè) >> 最新促銷 >> 144625-HRP Rabbit Anti-EIF2AK4抗體說(shuō)明

144625-HRP Rabbit Anti-EIF2AK4抗體說(shuō)明

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144625-HRP Rabbit Anti-EIF2AK4抗體說(shuō)明

 

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uSBIo品牌144625-HRP Rabbit Anti-EIF2AK4抗體實(shí)驗(yàn)說(shuō)明

144625-HRP

144625-HRP Rabbit Anti-EIF2AK4 (GCN2, KIAA1338, GCN2-like protein, Eukaryotic translation initiation factor 2-alpha kinase 4) (HRP)

EIF2AK4(真核翻譯起始因子2-Alpha激酶4),也稱為GCN2,是人類中由EIF2AK4基因編碼的酶。 EIF2AK4屬于激酶家族,其磷酸化真核翻譯起始因子-2的α亞基以響應(yīng)不同的細(xì)胞應(yīng)激下調(diào)蛋白質(zhì)合成。 Hartz(2005)基于EIF2AK4序列與基因組序列的比對(duì)將EIF2AK4基因定位于染色體15q15.1。 Berlanga等。 (1999)證明從小鼠肝臟提取物免疫純化的Gcn2可以在體外磷酸化兔Eif2。在用小鼠Eif2ak4轉(zhuǎn)染的人胚腎細(xì)胞中,血清饑餓使磷酸化EIF2-α的水平增加超過(guò)2倍。 Costa-Mattioli等。 (2005)報(bào)道了來(lái)自Gcn2缺失小鼠的海馬切片的*特征:在CA1中,單個(gè)100-Hz訓(xùn)練誘導(dǎo)強(qiáng)烈且持續(xù)的長(zhǎng)期增強(qiáng)(晚期LTP或L-LTP),其依賴于轉(zhuǎn)錄和翻譯。 

 

144625-HRP Rabbit Anti-EIF2AK4抗體說(shuō)明

 

EIF2AK4 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 4), also called GCN2, is an enzyme that in humans is encoded by the EIF2AK4 gene. EIF2AK4 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 to downregulate protein synthesis in response to varied cellular stresses. Hartz (2005) mapped the EIF2AK4 gene to chromosome 15q15.1 based on an alignment of the EIF2AK4 sequence with the genomic sequence. Berlanga et al. (1999) demonstrated that Gcn2 immunopurified from mouse liver extracts could phosphorylate rabbit Eif2 in vitro. Serum starvation increased the level of phosphorylated EIF2-alpha more than 2-fold in human embryonic kidney cells transfected with mouse Eif2ak4. Costa-Mattioli et al. (2005) reported a unique feature of hippocampal slices from Gcn2-null mice: in CA1, a single 100-Hz train induced a strong and sustained long-term potentiation (late LTP or L-LTP), which was dependent on transcription and translation.

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions

Western Blot: 0.1-0.5ug/ml. The detection limit for EIF2AK4 is ~0.5ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.

Storage and StABIlity

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: Applications are based on unconjugated antibody.

Immunogen

Synthetic peptide corresponding aa870-888, ATDHLAFSADSKQDDQTGD in the middle region of human EIF2AK4 (Q9P2K8)

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horserADIsh peroxidase (HRP).

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human h.EIF2AK4. No crossreactivity with other proteins.

 

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